Isolation and characterization of calf liver thioredoxin.

Isolation and characterization of a bovine herpesvirus 3 From cases of calf pneumonia in Iran

Bovine thioredoxin system. Purification of thioredoxin reductase from calf liver and thymus and studies of its function in disulfide reduction.

Thioredoxin reductase (EC l&4.5), which catalyzes the reduction of the disulfide bridge in thioredoxin by NADPH, was purified from calf liver and thymus. Preparation methods, involving chromatography on DEAE-cellulose, TEAEcellulose, and Sephadex G-200 or G-100 were used to purify the calf liver enzyme llOO-fold and the thymus enzyme 2800fold. The enzyme was shown to catalyze an NADPH-dependent...

Selenodiglutathione is a highly efficient oxidant of reduced thioredoxin and a substrate for mammalian thioredoxin reductase.

Selenium compounds like selenite (SeO3(2-) may form a covalent adduct with glutathione (GSH) in the form of selenodiglutathione (GS-Se-SG), which is assumed to be important in the metabolism of selenium. We have isolated GS-Se-SG and studied its reactions with NADPH and thioredoxin reductase from calf thymus or with thioredoxin reductase and thioredoxin from Escherichia coli. Incubation of 0.1 ...

Thioredoxin and glutaredoxin: small multi-functional redox proteins with active-site disulphide bonds.

Thioredoxin and glutaredoxin are small proteins with a redox-active disulphide/dithiol in the active site (Holmgren, 1985). Both exist in Escherichia colias well as in mammalian cells, and were originally isolated as hydrogen donors for ribonucleotide reductase (Holmgren, 1986). This essential enzyme is required in all cells to produce deoxyribonucleotides, which are the precursors for synthesi...

Protein disulphide-isomerase: a homologue of thioredoxin implicated in the biosynthesis of secretory proteins.

and have a tendency to aggregate, indicating that formation of a second disulphide in the molecule is accompanied by denaturation of the structure. Reduction of oxidized thymus thioredoxin can be achieved by dithiothreitol or by NADPH and thioredoxin reductase representing a possible autocatalytic control mechanism. Another major difference between the mammalian and the E. coli thioredoxin syst...